Coding

Part:BBa_K1758390

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-22)


His-tagged E. coli tryptophanase under control of the T7 promoter

This part can be used for expression and purification of the E. coli tryptophanase. A tryptophanase catalyzes the reaction of L-tryptophan and water to indole, pyruvate, and NH3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 638
    Illegal AgeI site found at 1259
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None